Recent Projects

In this study AppRidge was asked to determine if passage of a protein through a device caused the protein bioactivity to change. Changes in bioactivity were monitored by studying protein binding using BioLayer Interferometry (BLI). Through this study we concluded that the bioactivity of the protein as measured by BLI binding studies did not change after passage through the device. 

AppRidge was asked to analyze up to 19 sugars using up to 9 different technologies including GPC/MALLS/RI, NMR, LC/MS, TGA, ICP/MS, particle size, DSC, microbial testing and endotoxin testing. Each of these tests was performed by one of our external partner laboratories.  The customer was provided results from each test accomplished on each sugar with a comparison to the sugar from their competitor.

AppRidge reviewed several technical reports on the development of an analytical test provided by a 3rd party laboratory to a client and provided critical comments on various aspects of the reports. In addition, we examined technology under consideration by a client for preparation of a carbohydrate product with very specific physical requirements.  AppRidge presented concerns about the technology and an alternate approach  to the project goal for consideration by the client.

AppRidge investigated production opportunities for a client wishing to isolate a specific carbohydrate.  A suitable production site was found and discussion began.  However, the client decided to drop the project for internal reasons not related to the production site.  In another project, AppRidge reviewed a production process for a client and provided comments and suggestions on the process.

AppRidge provided a client with a compositional analysis of a fiber sample using proton NMR  for confirmation of its identity.  The fiber was found to contain 3 different monomers. Relative amounts of each monomer were provided to the client.  In addition, a fiber characterization using NMR was accomplished to determine the presence or absence of an impurity.

AppRidge has examined structural changes in fungal cell wall glucans and mannans for several clients using proton 1D and 2D NMR, GPC/MALLS/RI and GC/MS.  Several NMR techniques were employed including DOSY for purity characterization and selective 1D TOCSY as well as 2D COSY, HSQC, HMBC, TOCSY and NOESY for structural characterization.  These analyses enable a detailed structure/property relationship characterization to enable further consideration for various biomedical applications.