Analytical Technologies

Proton and multinuclear solution-state 1D and 2D NMR from 600 to 800 MHz and solid-state NMR at 360 MHz. NMR is the most powerful analytical technique available today for the structural characterization of complex carbohydrates.  

Gel Permeation Chromatography (GPC) with Multi-Angle Laser Light Scattering (MALLS) detection and refractive index (RI) detection provides a measure of the carbohydrate polymer molecular weight, polydispersity and solution conformation. 

Gas Chromatography/Mass Spectrometry (GC/MS) for linkage, monomer type and monomer substitution analysis based upon permethylated alditol chemistry.

Bio-Layer Interferometry (BLI) is an optical biosensing technology that analyzes biomolecular interactions in real-time without the need for fluorescent labeling.  BLI allows for a higher volume of information to be obtained in a quicker amount of time compared to traditional processes. BLI is applicable to the quantification of carbohydrate ligand-receptor binding, protein interactions, and properties of reaction kinetics, such as rate constants and reaction rates.

Endotoxin testing looks for bacterial endotoxins, members of a class of phospholipids called lipopolysaccharides (LPS).  LPS are found in the outer membrane of gram-negative bacteria and are released after death and lysis of the bacterial cell. The presence of LPS can impact adversely biological testing.