Structural Characterization of Mannans using Proton NMR

The structural features incorporated in the main (1-6)-linked backbone chain, the acid-stable side chains attached to the backbone chain and the acid-labile phosphate-linked side chains are characterized by 1D proton NMR and 2D COSY NMR. The side chains can be composed of α- and  ß-linked  (1-2)- and (1-3)-glycosidic linkages. Based upon NMR analysis, it is straight-forward to characterize mannan structural features and determine changes in the amount of these structural features by comparison with a reference mannan spectrum or one of the mannan spectra in your study. We have characterized  structures of mannans isolated from Saccharomyces cerevisiae (1), Candida albicans (1,2), C. glabrata (3) and C. auris (4).

Selected References:

(1) "Dendritic Cell Interactions with Candida albicans Critically Depends on N-Linked Mannan," A. Cambi, M. G. Netea, H. M. Mora-Montes, N. A. Gow, S. V. Hato, D. W. Lowman, B.-J. Kullberg, D. L. Williams, C. G. Figdor, Journal of Biological Chemistry, 283, 20590-20599 (2008)" 

(2) “Mannan structural complexity is decreased when Candida albicans is cultivated in blood or serum at physiological temperature,” Douglas W. Lowman, Harry E. Ensley, Rachel R. Greene, Kevin J. Knagge, David L. Williams, Michael D. Kruppa, Carbohydrate Research 2011, 346, 2752-2759

(3) West, Lara, Lowman, Douglas W., Mora-Montes, Hector M., Grubb, Sarah, Murdoch, Craig, Thornhill, Martin H., Gow, Neil A. R., Williams, David, and Haynes, Ken, “Differential virulence of Candida glabrata glycosylation mutants,” Journal of Biological Chemistry, 288(30), 22006-18, 2013.

(4) Mariolina Bruno, Simone Kersten, Judith M. Bain, Martin Jaeger, Diletta Rosati, Michael D. Kruppa, Douglas W. Lowman, Peter J. Rice, Bridget Graves, Zuchao Ma, Y. Ning Jiao, Anuradha Chowdhary, George Renieris, Frank L. van de Veerdonk, Bart-Jan Kullberg, Evangelos, J. Giamarellos-Bourboulis, Alexander Hoischen, Neil A. R. Gow, Alistair J. P. Brown, Jacques F. Meis, David L. Williams, Mihai G. Netea, "Transcriptional and functional insights into the host immune response against the emerging fungal pathogen Candida auris". Nature Microbiology, August 24 (2020) 1-16, https://doi.org/10.1038/s41564-020-0780-3