Physico-Chemical and Structural Characterization Capabilities

  • Nuclear Magnetic Resonance Spectroscopy (NMR)
    • 1H and multinuclear solution-state 1D and 2D NMR from 400 to 950 MHz for carbohydrate structure characterization and purity analysis in isolated samples as well as complex biomatrices.
    • Multinuclear solid-state NMR

  • Gas Chromatography/Mass Spectrometry (GC/MS) based upon either of the following techniques:
    • Permethylated alditol chemistry for linkage analysis, monomer type analysis and monomer substitution.
    • Enzymatic degradation for analysis of primary and secondary sidechain linkages, length and compositions of sidechains and branch points.
  • Electrospray Mass Spectrometry (ES-MS)

  • Matrix Assisted Laser Desorption Ionization - Time-of-Flight Mass Spectrometry (MALDI-TOF-MS)

  • Multi-detector Gel Permeation Chromatography (GPC) using Multi-Angle Laser Light Scattering (MALLS), Differential Viscometry (DV) and Refractive Index (RI) detection systems

  • Surface Plasmon Resonance Spectroscopy (SPR) for carbohydrate ligand-receptor binding studies
  • Size Exclusion Chromatography (SEC) to narrow the molecular weight range, or ranges, of isolated carbohydrates obtained from a complex matrix
  • Atomic Force Microscopy (AFM)
  • Scanning Electron Microscopy (SEM)
  • Optical Spectroscopy including IR, Raman, NIR and UV/Vis
  • Optical Microscopy including IR and Raman
  • X-Ray Diffraction (XRD)
  • Protein Identification
  • Endotoxin testing